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20-30 hours’ preparation before the ASCP-MLT exam
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ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:
1. Convert the following temperature from Celsius to Fahrenheit
20 degrees C
Question options:
A) 77 degrees F
B) 39.2 degrees F
C) 68 degrees F
D) 14 degrees F
2. Hemoglobin electrophoresis uses an electric field to separate hemoglobin molecules based on differences in net electrical charge. The rate of electrophoretic migration is also dependent on the ionic radius of the molecule, the viscosity of the solution through which it is migrating, the electrical field strength, temperature, and the type of supporting medium used.
Electrophoretic separation of hemoglobin fundamentally relies on:
A) Concentration differences of molecules
B) Size differences of molecules
C) Net charge differences of molecules
D) Shape variations of molecules
3. A laboratory scientist is preparing a chemical reagent for testing and accidentally spills the chemical on the counter. How is this contamination taken care of?
A) The laboratory scientist can ask a co-worker to assist in the cleanup of the spill.
B) The laboratory scientist can just wipe the spill with saline and throw the towel in the garbage.
C) The laboratory scientist needs to consult with the safety data sheets (SDSs).
D) The laboratory scientist must consult with their supervisor on the proper way to clean the spill.
4. Considering the reactions given in this case study, Yersinia enterocolitica would be the best choice.
Most strains of Plesiomonas shigelloides, Escherichia coli, and Pasteurella multocida are urease negative.
Also, Plesiomonas shigelloides demonstrates a K/A reaction on a TSI slant.
The stool specimen from a patient admitted to the hospital with symptoms of appendicitis has a culture with the following characteristics:
Gram-negative bacillus
Catalase positive
Urease positive
Weakly fermentative
TSI Slant is A/A
These reactions suggest that the organism is MOST likely:
A) Pasteurella multocida
B) Yersinia enterocolitica
C) Escherichia coli
D) Plesiomonas shigelloides
5. The MCV is indicative of microcytosis with MCV=<80fL. The RDW is within normal limits and indicative of a homogenous cell population.
If the MCV was >100 fL, this would be indicative of macrocytosis. An RDW that was outside of normal limits would be indicative of a heterogenous cell population.
An 18 year old female has a CBC as part of a routine physical exam. The following results were obtained and the physician determines she is anemic. After reviewing her CBC results shown below, which of the following would be an appropriate description of the anemia?
White blood cells (WBC): 5.6 x 10^9/L (RR:4.0-10.0 x 10^9/L)
Red blood cells (RBC): 3.7 x 10^12/L (RR: 4.2-5.9 x 10^12/L)
Hemoglobin: 9.9 g/dL (RR:12-16 g/dL)
Hematocrit: 28% (RR: 37-48%)
MCV: 75 fL (80-100 fL)
RDW-CV: 14% (RR: 11.0-15.0%)
A) Microcytic, homogenous
B) Macrocytic, homogenous
C) Macrocytic, heterogenous
D) Microcytic, heterogenous
Solutions:
| Question # 1 Answer: C | Question # 2 Answer: C | Question # 3 Answer: C | Question # 4 Answer: B | Question # 5 Answer: A |





